Chromatography is a laboratory process of separating the required components of a mixture or a solution. Chromatography vials, also known as GC vials, HPLC vials, and ion chromatography vials, play a crucial role in the entire process. The vials are chosen on the basis of the type of process, samples to be analysed, and more. For this blog, let us focus on understanding chromatography vials a bit better.
What are Chromatography Vials?
Chromatography vials are containers used to fill samples in the GC (Gas Chromatography) or LC (Liquid Chromatography) analysis. You can easily source these vials from a chromatography lab vial supplier.
What Material is Chromatography Vials Made Of?
Chromatography vials are generally made of plastic or clear glass. The amber-tinted vials protect the samples from direct exposure to light. Glass is chosen because of its inertness. In contrast, polymers might be needed to get rid of interferences during ion chromatography.
The most popular plastic options are polypropylene (PP) and polymethylpentene (PMP). Both the plastic materials are transparent; PP can resist heat up to 135 degrees Celsius, whereas PMP can resist heat up to 175 degrees Celsius.
What is the Standard Size of the Chromatography Vial?
The typical sizes of chromatography vials include 8x40mm, 15x45mm, and 12x32mm. Robotic arm machines generally use a 9mm non-standard vial featuring a magnetic cap. Inserts are used to reduce the vial capacity while maintaining the external dimensions. They are typically used for smaller samples and may be conical, bottomed, or pre-fused in the vial. Ensure to choose insert according to the vial dimensions.
Features of Chromatography Vials
Some of the standard features of chromatography vials are:
- Frosted writing areas
- Rounded or flat bottoms
- Graduations
- Normal or wide–mouth openings
- Thick wall to withstand vacuum or pressure
Even though it is possible to reuse the closures and vials sometimes, it is not recommended as it increases the chances of contamination from atmospheric or solvent interactions, ultimately affecting the result and raising the need for re-work.
How Do You Choose Septa?
Septa and liner in chromatography vials play a crucial role in the preparation and storage of samples. When choosing septa, check its compatibility with the solvent. The majority of septa are faced with PTFE, and when the polytetrafluoroethylene (PTFE) layer is punctured, there are chances the solvent will come in exposure to the material.
How Do You Choose the Correct Chromatography Vial?
Choosing the correct chromatography vial depends on several factors; however general steps to consider are:
- Determine the closure style of the vial
- Decide on the vial volume
- Choose appropriate material from glass, plastic, or other based on the requirement
- Select whether you want amber or clear vial
Wrapping Up
We hope you now know chromatography vials used in laboratories a bit better. If you or any of your friends are looking forward to ordering these vials, get in touch with a chromatography vials supplier in USA to source quality vials.
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